Blar i forfatter "Dawadi, Rangita"
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Biochemical characterization of Matrix Metalloproteinase-9 Binding interactions with two small non-peptide inhibitors and the proteoglycan serglycin & Processing of serglycin
Dawadi, Rangita (Doctoral thesis; Doktorgradsavhandling, 2020-04-17)The present work has a focus on biochemical characterization of the human matrix metalloproteinase-9. First part of the investigation focuses on the enzymes ability to form a complex with the small proteoglycan serglycin. Here the focus was on which amino acids and regions in both proMMP-9 and serglycin are important for the complex formation. For that, recombinant full-length proMMP-9 and various ... -
In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
Dawadi, Rangita (Master thesis; Mastergradsoppgave, 2014-05-15)Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ... -
Molecular Interactions Stabilizing the Promatrix Metalloprotease-9·Serglycin Heteromer
Dawadi, Rangita; Malla, Nabin; Hegge, Beate; Wushur, Imin; Berg, Eli; Svineng, Gunbjørg; Sylte, Ingebrigt; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-12)Previous studies have shown that THP-1 cells produced an SDS-stable and reduction-sensitive complex between proMMP-9 and a chondroitin sulfate proteoglycan (CSPG) core protein. The complex could be reconstituted in vitro using purified serglycin (SG) and proMMP-9 and contained no inter-disulfide bridges. It was suggested that the complex involved both the FnII module and HPX domain of proMMP-9. The ... -
The selectivity of galardin and an azasugar-based hydroxamate compound for human Matrix metalloproteases and bacterial metalloproteases
Sylte, Ingebrigt; Dawadi, Rangita; Malla, Nabin; von Hofsten, Susannah; Nguyen, Tra-Mi; Solli, Ann Iren; Berg, Eli; Adekoya, Olayiwola A.; Svineng, Gunbjørg; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-08-03)Inhibitors targeting bacterial enzymes should not interfere with enzymes of the host, and knowledge about structural determinants for selectivity is important for designing inhibitors with a therapeutic potential. We have determined the binding strengths of two hydroxamate compounds, galardin and compound 1b for the bacterial zinc metalloproteases, thermolysin, pseudolysin and auerolysin, known to ...